Figure 4. The TRPV1 protein level in the keratinocytes was under detection limit in Western blotting assay.

Proteins were extracted from human trigeminal ganglion (TG), primary human keratinocytes (NEHK) and the HaCaT cell lines. With a commercially available polyclonal antibody only the TG expressed 94 kDa TRPV1 can be detected (A). The equal loading of protein extracts was validated by β-actin (∼50 kDa), a common, ubiquitously expressed cytoskeletal protein (B).