Figure 1.

Formation of Y-shaped oligodeoxynucleotide (Y-ODN). (a) Putative structure of Y-ODN, which was prepared with three ODNs (Y0a, Y0b, Y0c) with the halves of each ODN being partially complementary to a half of the other two ODNs. (b) Polacrylamide gel electrophoresis analysis of single-stranded (ss), double-stranded (ds) and Y-ODN. Each ODN was run on 21% polyacrylamide gel at 200 V for 2 hr. Lane 1, 20-bp DNA ladder (Takara Bio); lane 2, ssODN; lane 3, dsODN; lane 4, Y-ODN; lane 5, Y-ODN(CpG); lane 6, Y-ODN(CpG₃).