Figure 10.

Dominant active RhoA I117 does not abrogate the cytoskeletal alterations induced by myr 5 expression. HtTA-1 HeLa cells were transfected with pUHD10–3 myc-RhoA I117 alone (a and b) or together with pUHD10–3 myr 5-HA (c–f). Cells were processed for immunofluorescence 40 h after transfection. Double immunofluorescence staining for myc-RhoA I117 (a) with antibody 9E10 followed by a secondary antibody coupled to lissamine rhodamine and F-actin (b) with FITC-phalloidin was performed in cells transfected with myc-RhoA I117. Triple immunofluorescence staining was performed for cells cotransfected with myc-RhoA I117 and myr 5-HA. Myc-RhoA I117 (c) was stained with antibody 9E10 followed by a secondary antibody coupled to lissamine rhodamine; myr 5-HA (d) was stained with antibody Tü 66 followed by a secondary antibody coupled to AMCA; and F-actin (e) was stained with FITC-phalloidin. (f) Cells are shown in phase-contrast optics. Bar, 20 μm.