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Localization of endogenous myr 5 in NRK cells. NRK cells were fixed directly (a, b, e, and f) or after permeabilization with Streptolysine-O (c and d) to remove cytosol and processed for double immunofluorescence. myr 5 was stained with antibody Tü 66 (a and e) or antibody Tü 52 (c) followed by a secondary antibody coupled to Texas red and F-actin (b, d, and f) was stained with FITC-phalloidin. The cell in e and f is undergoing cytokinesis. Bars, 10 μm.
