Figure 5.

Expression of the different myr 5 constructs in baculovirus-infected Sf9 cells. Sf9 cells were infected with recombinant baculovirus encoding full-length myr 5 (myr 5), the myr 5 head domain (head), the myr 5 tail domain (tail), or the myr 5 Rho-GAP domain (GAP). Cells were lysed 12, 24, 36, 48, and 63 h after infection, respectively, and prepared for SDS-PAGE. Coomassie blue-stained gels are shown. Molecular mass markers are indicated on the left; time after infection is indicated above each lane and bands corresponding to the respective myr 5 constructs are marked on the right.