Figure 6.

Rho-GAP activity in lysates of cells infected with recombinant baculovirus encoding different myr 5 constructs. Purified RhoA was preloaded with radioactive GTP and incubated at 30°C with 6 μg of protein from lysates of cells infected for 48 h. After 15 min, the remaining GTP was determined in a filter-binding assay. As a control, RhoA was incubated with lysis buffer alone (intrinsic) and the remaining GTP was set 100%. Lysates of cells expressing myr 5 (myr 5), myr 5 tail domain (tail), or the myr 5 Rho-GAP domain (GAP) exhibit increased Rho-GAP activity as compared with lysates of cells expressing the myr 5 head domain (head) or uninfected cells (ni).