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. 1997 Oct;8(10):2039–2053. doi: 10.1091/mbc.8.10.2039

Figure 8.

Figure 8

Rho-GAP activity of myr 5 is responsible for the observed alterations in cytoskeletal organization. HtTA-1 HeLa cells were transfected with plasmids pUHD10–3 myr 5-HA (a–c), pUHD10–3 HA-myr 5 Rho-GAP domain (d–f), and pUHD10–3 myr 5-HA M1695 (g–i). The cells were stained 40 h after transfection for myr 5 with antibody Tü 66 (a and g) or hemagglutinin antibody HA.11 (d) followed by a secondary antibody coupled to lissamine rhodamine and for F-actin (b, e, and h) by using FITC-phalloidin. The identical fields of cells are also shown in phase-contrast optics (c, f, and i). Bars, 20 μm.