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Effect of lipid mixtures on calcein flux through MscL. The G22C mutant of E. coli MscL was reconstituted into lipid vesicles containing the following lipid mixtures: (a) DOPC:DOPG: CL (70:25:5 mol %), (b) DOPE:DOPG:CL (70:25:5 mol %), and (c) azolectin. After 50 s, 1 mM MTSET was added, followed finally by 200 μM C12E8 to burst the vesicles.
