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. Author manuscript; available in PMC: 2008 Oct 13.
Published in final edited form as: Biochemistry. 2008 Mar 15;47(14):4317–4328. doi: 10.1021/bi702409t

Figure 8.

Figure 8

Calcein flux following reaction of MscL with MTSES. The G22C mutant of E. coli MscL was reconstituted into lipid vesicles containing DOPC and the given mole percent DOPG. After 50 s, 1 mM MTSES was added, followed finally by 200 μM C12E8 to burst the vesicles.