Figure 1.
SDS-PAGE gel: Lane 1: M.W. markers; Lane 2: soluble P. pastoris cell lysate; Lane 3: IMAC purified aspartoacylase; Lane 4: purified aspartoacylase after ion exchange chromatography.
SDS-PAGE gel: Lane 1: M.W. markers; Lane 2: soluble P. pastoris cell lysate; Lane 3: IMAC purified aspartoacylase; Lane 4: purified aspartoacylase after ion exchange chromatography.