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Kinetics of aspartoacylases expressed in E. coli and P. pastoris
| substratea | parameters | E. coli-expressed murine aspartoacylase | P. pastoris-expressed human aspartoacylase |
|---|---|---|---|
| X = –CH3 | kcat (s−1) | 0.083 ± 0.006 | 12. 7 ± 0.05 |
| Km (mM) | 0.36 ± 0.06 | 0.12 ± 0.03 | |
| X = –CF3 | kcat (s−1) | 1.2 ± 0.09 | 116 ± 13 |
| Km (mM) | 0.21 ± 0.04 | 0.15 ± 0.05 |
parent compound:
