Table 2.
Steady state ATPase parameters for rGyr_hel and rGyr_fl, and the influence of nucleic acid substrates
| Nucleic acid | kcat (10−3 s−1) | KM,DNA (μM) | KM (μM base/bp) | |
|---|---|---|---|---|
| rGyr_hel | – | 30 ± 2 | NA | NA |
| ssDNA | 1160 ± 188 | 0.07 ± 0.04 | 4.2 ± 2.4 | |
| dsDNA | 1450 ± 64 | 0.18 ± 0.03 | 10.8 ± 1.8 | |
| pUC18 | 673 ± 21 | 0.046 ± 0.006 | 124 ± 16 | |
| polyU-RNA | 3264 ± 304 | NA | 25 ± 10 | |
| Heparin | 2420 ± 160 | 0.20 ± 0.05 | 8.0 ± 0.04 | |
| rGyr_fl | – | 20 ± 0.8 | NA | NA |
| ssDNA | 160 ± 6.7 | 0.45 ± 0.06 | 27 ± 4 | |
| dsDNA | 148 ± 9.1 | 2.2 ± 0.30 | 129 ± 18 | |
| pUC18 | No saturation for conc. <400 nM | |||
| polyU-RNA | 507 ± 27 | NA | 30 ± 7 | |
| Heparin | 120 ± 10 | 0.15 ± 0.06 | 6 ± 2.4 | |