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Steady state ATPase parameters for rGyr_hel and rGyr_fl: the influence of nucleic acid substrates on KM,ATP
| Nucleic acid (saturating) | kcat (10−3 s−1) | KM,ATP (μM) | |
|---|---|---|---|
| rGyr_hel | – | 30 ± 2 | 77 ± 23 |
| ssDNA | 992 ± 12 | 4.8 ± 1.0 | |
| dsDNA | 1435 ± 57 | 11 ± 4 | |
| pUC18 | 818 ± 10 | 7.4 ± 1.1 | |
| polyU-RNA | 3402 ± 79 | 42 ± 5.5 | |
| Heparin | 2869 ± 63 | 31 ± 4 | |
| rGyr_fl | – | 20 ± 0.8 | 44 ± 6 |
| ssDNA | 108 ± 2.1 | 12 ± 1.5 | |
| dsDNA | 117 ± 1.6 | 16 ± 1.5 | |
| pUC18 (300 nM) | 199 ± 1.6 | 15.6 ± 0.9 | |
| polyU-RNA | 565 ± 19 | 32 ± 5.1 | |
| Heparin | 133 ± 7 | 37 ± 6.7 |
