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. 2008 Sep 16;36(18):5882–5895. doi: 10.1093/nar/gkn587

Table 4.

DNA binding properties of different nucleotide states of rGyr_hel

K_d (μM)	nucleotide-free	ADP state		ATP state
ssDNA
wt	0.20 ± 0.03^a	ADP	0.31 ± 0.04^a	ADPNP	0.60 ± 0.07^a
	0.20 ± 0.01^b		0.28 ± 0.01^b		0.46 ± 0.03^b
	n = 1.4 ± 0.1		n=1.6 ± 0.1		n=1.4 ± 0.1
		ADPCP	0.38 ± 0.05^a	ATPγS	1.2 ± 0.2^a
			0.33 ± 0.01^b		0.92 ± 0.21^b
			n=1.6 ± 0.1		n=1.2 ± 0.2
K106Q	0.18 ± 0.03^a		0.25 ± 0.03^a		0.19 ± 0.02^a
	0.18 ± 0.03^b		0.24 ± 0.02^b		0.20 ± 0.02^b
	n = 1.3 ± 0.2		n=1.3 ± 0.1		n=1.1 ± 0.1
dsDNA
wt	3.9 ± 0.6 ^a	ADP	3.7 ± 0.5^a	ADPNP	0.19 ± 0.03^a
		ADPCP	4.2 ± 0.3^a	ATPγS	1.0 ± 0.1 ^a
K106Q	3.8 ± 0.6 ^a		2.9 ± 0.6 ^a		1.7 ± 0.2 ^a

^aOne-site.

^bHill analysis.