Skip to main content

View full-text article in PMC

. 2008 Sep 16;36(18):5882–5895. doi: 10.1093/nar/gkn587

Table 5.

DNA binding properties of different nucleotide states of rGyr_fl

K_d (μM)	nucleotide-free	ADP state		ATP state
ssDNA
Y851F	0.035 ± 0.005^a	ADP	0.060 ± 0.015^a	ADPNP	0.041 ± 0.009^a
	0.036 ± 0.001^b		0.045 ± 0.002^b		0.038± 0.002^b
	n = 2.3 ± 0.2		n = 2.1 ± 0.2		n = 2.1 ± 0.2
		ADPCP	0.049 ± 0.012^a	ATPγS	0.046 ± 0.008
			0.039 ± 0.001^b		0.041 ± 0.001^b
			n = 2.1 ± 0.1		n = 1.8 ± 0.1
K106Q/Y851F	0.047 ± 0.005^a		0.053 ± 0.007^a		0.037 ± 0.004^a
	0.044 ± 0.003^b		0.044 ± 0.002^b		0.035 ± 0.002^b
	n = 1.4 ± 0.1		n = 1.5 ± 0.1		n = 1.4 ± 0.1
dsDNA
Y851F	0.49 ± 0.06^a	ADP	0.25 ± 0.04^a	ADPNP	0.33 ± 0.07^a
		ADPCP	0.59 ± 0.06^a	ATPγS	0.53 ± 0.07^a
K106Q/Y851F	0.71 ± 0.11^a		0.46 ± 0.07^a		0.49 ± 0.09^a

^aone-site.

^bHill analysis.