Figure 6.

Expression of the antisense RNAs in C. acetobutylicum. Northern blot analysis was carried out on total RNA extracted from strain ATCC824 after growth with 1 mM methionine (M) or 1 mM cysteine (C). The antisense RNAs were detected with the probe hybridizing with the S-box motif (A) or the mccA gene (B). The positions of the probes are indicated by arrows on the corresponding genomic region scheme. Broken arrows with ‘P’ symbol indicate the position of the upstream and downstream promoters; loops indicate putative transcriptional terminators. The arrows show the different antisense RNAs detected with their estimated size. The RNA size is determined with single strand RNA size markers ‘RNA millennium’ (Ambion). These results are representative of at least two independent experiments.