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. 2008 Aug 18;36(18):e117. doi: 10.1093/nar/gkn523

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Screening ES clones targeted by a Tec/Rlk modified-BAC by FISH. (A) Schematic diagram of the BAC targeting construct in RP23 61-85. Location of the Tec gene is indicated in red. Location of the Rlk gene is indicated in blue. The locations of the respective mutations introduced in Tec and Rlk are indicated by the inserted neo and bsd genes, respectively. (B) Pulse field gel electrophoresis of (1) the BAC targeting construct (175 kb) digested with NotI is indicated with an arrow; (2) empty lane and (M) Lambda Ladder PFG Marker (New England BioLabs, Ipswich, MA, USA). (C, D) Representative FISH images using the BAC RPCI-23-6518 probe containing Tec and Rlk genes (red on chromosome 5). Two pairs of spots in chromosome 5 (white arrows) suggest homologous integration (C) whereas a third set of spots (yellow arrow) in another chromosome indicates non-homologous integration (D).