Table 2.
Comparison of results for individual ES clones electroporated with the Tec/Rlk-modified BAC
| ES selected with G418/blasticidin | RQ Tec | RQ Rlk | FISH screening |
|---|---|---|---|
| WT | 1.0 | 1.0 | |
| Positive Tec+/− | 0.6 | 1.1 | |
| Positive Rlk+/− | 1.2 | 0.5 | |
| GB 1 | 0.3 | 0.4 | 2 spots |
| GB 2 | 0.6 | 1.0 | 2 spots |
| GB 3 | 1.2 | 1.0 | 3 spots |
| GB 4 | 1.2 | 1.0 | 3 spots |
| GB 5 | 1.3 | 1.0 | 2 spots |
| GB 6 | 1.3 | 1.1 | 3 spots |
| GB 7 | 0.6 | 0.9 | 3 spots |
| GB 8 | 1.1 | 1.1 | 2 spots |
| GB 9 | 1.3 | 1.0 | 3 spots |
| GB 10 | 1.2 | 1.1 | 3 spots |
| GB 11 | 1.2 | 1.1 | 3 spots |
Summary of results for individual ES cells selected with both G418 and blasticidin and screened by q-PCR for deletion of Tec- and Rlk-targeted exons. Results are compared to those obtained by FISH using the BAC probe. Clones that are positive by q-PCR are in bold. Clones in red contained a gene-disruption of Tec. Clones indicated in blue contained a gene-disruption for Rlk. Results of FISH screening that are false positives are italicized.