Table 4.
Template | Gel assay | SAMS | 5′-NX′N-3′ | Number of mutants |
---|---|---|---|---|
5′-TFT | 63 | 68 | GGCTTCCT | 58 |
GGC TCCT | 121 | |||
5′-AFC | 22 | 17 | GGCACCCT | 70 |
GGC CGCC | 14 | |||
GGCA CCT | 0 | |||
5′-GFA | 64 | 81 | GGCGACCT | 24 |
GGC ACCT | 98 | |||
GGCG CCT | 0 | |||
5′-CFT | 8 | 8 | GGCCTCCT | 89 |
GGC TCCT | 8 | |||
GGCC CCT | 0 |