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Polymerization kinetics of purified complexes (see Materials and Methods section). DNA-synthesis rates (v, nucleotides per second) were determined for various nucleotide substrate concentrations (s, micromolar). (A) Purified complexes: Km = 54 μM, Vmax = 3.4 nt/s. (B) Control sample of uncomplexed polymerase and DNA: Km = 21 μM, Vmax = 12.0 nt/s.
