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Concentration dependence on dipolar coupling. (A). EPR spectrum of oligomer IX in PNE buffer at −10°C. Arrows show additional features in the spectrum that are consistent dipolar coupling between two nitroxides resulting from helical stacking in solution. Unlabeled duplex of the same sequence was added to this sample; 1, 4 and 10 equivalents, shown in (B, C and D, respectively).
