Table 1.

T_Ms of DNA duplexes I–XI containing Ç^a

Duplex	Sequence	TMb	ΔTMc
I	5′-d(GTGCGCAC)	49.5 ± 0.5
	3′-d(CACGCGTG)
II	5′-d(GTGCGÇAC)	46.6 ± 0.6	−2.9
	3′-d(CAÇGCGTG)
III	5′-d(GTGÇGCAC)	46.3 ± 0.5	−3.2
	3′-d(CACGÇGTG)
IV	5′-d(CGCGAATTCGCG)	57.4 ± 0.5
	3′-d(GCGCTTAAGCGC)
V	5′-d(CGCGAATTÇGCG)	63.1 ± 0.5	+5.7
	3′-d(GCGÇTTAAGCGC)
VI	5′-d(GACCTCGCATCGTG)	62.8 ± 0.3
	3′-d(CTGGAGCGTAGCAC)
VII	5′-d(GACCTCGÇATCGTG)	61.7 ± 0.6	−1.1
	3′-d(CTGGAGCGTAGCAC)
VIII	5′-d(CACCTCGCATCGTG)	65.4 ± 0.3
	3′-d(GTGGAGCGTAGCAC)
IX	5′-d(ÇACCTCGCATCGTG)	67.3 ± 0.2	+1.9
	3′-d(GTGGAGCGTAGCAC)
X	5′-d(ACCTCGCATCGTG)	60.7 ± 0.3
	3′-d(TGGAGCGTAGCAC)
XI	5′-d(ÇACCTCGCATCGTG)	65.3 ± 0.4	+4.6
	3′-d(TGGAGCGTAGCAC)

^a2 μM duplex in 10 mM sodium phosphate, 100 mM NaCl, 0.1 mM Na₂EDTA, pH 7.0.

^bT_Ms of duplexes (°C).

^cΔT_M is the difference in the T_M values between a duplex having Ç or C in a particular sequence.