Table 1.

Predicted function of the mitochondrial proteins identified after anti-FLAG mtRRF affinity precipitation

Protein typea	Immunoprecipitationhr
	mtRRFb	mtRRF + EDTAc	mtRRF + RNased
Mitoribosomal proteinse	73	1	0
Nucleoidf
Class I	25 (31)	2	0
Class II	6 (10)	0	0
Class III	6 (16)	0	0
Nucleoidg	5 (6)	1	0
Translation associated	6	1h	1h
Nucleic acid binding/modifying	13	0	0
Metabolic	23	0	0
Chaperone/protease	5	0	0
Transporter	4	0	0
Unknown function	6	0	0
TOTAL	167j	7i	1

^aKnown or predicted function of identified mitochondrial proteins.

^bImmunoprecipitation from mitoplasts after protease and DNase shaving.

^cEDTA-washed immunoprecipitant from crude mitochondria.

^dRNase-treated immunoprecipitant from crude mitochondria.

^eMitoribosomal subunits identified by Koc et al. 2001 (39) and Cavdar Koc et al. (40).

^fPutative nucleoid proteins as recently classified by Bogenhagen et al. (25) (of total proteins).

^gPutative nucleoid proteins as identified by He et al. (26) (of total proteins).

^hMitochondrial ribosomal recycling factor, mtRRF.

ⁱThree cytosolic ribosomal proteins were identified in the precipitation following EDTA treatment.

^jDoes not include any non-mitochondrial, hypothetical or predicted proteins that were found.