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. 2008 Sep 6;36(18):5727–5735. doi: 10.1093/nar/gkn567

Figure 3.

Figure 3.

Promoter analysis of the miR-433 and miR-127 luciferase reporters. (A) Transient transfection assays to determine nuclear receptor regulation of miR-433 and miR-127 promoter (Pro.) transactivation. The promoters of pri-miR-433 and pri-miR-127 were cloned into a pGL3-basic vector, respectively. Hela cells were transfected with the miR-433Luc or miR-127Luc in the presence of various nuclear receptor expression plasmids (200 ng). Luciferase (Luc.) activities (act.) were determined, which were normalized by β-gal activities. Con, control (pcDNA3); R, RXRa; L, LXRa; F, FXR; T, TRα; E, ERα; C, CAR; Pα, PPARα; Pβ, PPARβ; Pγ, PPARγ; Eα, ERRα; Eγ, ERRγ. (B) Dose-dependent transactivation of miR-433 and miR-127 promoter (Pro.) reporters by ERRγ (50, 100 and 200 ng), which were inhibited by SHP (50, 100 and 200 ng). (C) Deletion inactivation of the putative ERRE sites in the miR-433 (E1 and E2) and miR-127 (E1′ and E2′) promoter reporters. Each deletion reporter was transfected with the ERRγ expression plasmid (white bar, 20 ng; black bar, 40 ng) and luciferase activities were determined and normalized.