Figure 4.

(A) ChIP analysis of ERRγ co-immunoprecipitation (Co-IP) on the miR-433 and miR-127 promoter region containing putative ERRE sites. The endogenous promoters in Hepa-1 cells were analysed. RNAi, ERRγ knockdown; non-specific (n.s.), using primers ∼11 kb downstream of miR-127 promoter which served as negative control. The ERRE in ERRα promoter served as positive control (pos. con.). The n.s. primers were located ∼10 kb upstream of the ERRα promoter. Nmuli cells were used. (B) Gel shift analysis to determine specific ERRγ protein binding to the putative ERRE sites in the miR-433 and miR-127 promoters. Comp, competitor; mut, mutant probe; cold, cold probe (10×).