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. 2008 Sep 6;36(18):5750–5762. doi: 10.1093/nar/gkn553

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Analysis of dsDNA RSS breaks in WT and cR2 thymocytes sorted based on DNA content (2C, 4C). Nuclei from several (pooled) 1-month-old mouse thymi were stained with PI and sorted into 2C (G0/G1) and 4C (G2/M) populations. Genomic DNA was subjected to LM-PCR to detect RSS breaks at the indicated antigen receptor gene segment RSSs. The negative image of an ethidium-stained agarose gel is shown. 63-12 is a RAG2-null cell line whose DNA served as a negative control. The direct amplification of β-globin serves as a DNA-loading control.