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. Author manuscript; available in PMC: 2008 Oct 23.
Published in final edited form as: Biochemistry. 2007 Oct 2;46(42):11718–11726. doi: 10.1021/bi700894h

Figure 4. Binding of L-CaM to IQ12 and IQ23 as determined by FRET.

Figure 4

FRET emission intensity (λex = 295 nm, λem = 490 nm) of 0 – 3 μM L-CaM and (top) 0.5 μM IQ12 and (bottom) 0.5 μM IQ23 in the (●) absence and (■) presence of 100 μM free calcium. Lines are drawn by eye and show stoichiometric binding of calmodulin to the IQ motifs.