Table 1.
Experimental approaches used in this study
Separation | Mass Spectrometry | Label | Virus/Target | Purpose | Time points (hours) | Figure |
---|---|---|---|---|---|---|
1D-nano LC/MS | ESI-ion trap | None | Measles virus proteins | Viral protein expression levels | 0, 18, 72 | 1 |
1D-capillary LC/MS | ESI-TOF | None | Measles virus proteins | Relative protein quantitation | 0, 18, 72 | 1 |
None | DIOS | 16O/18O | FHV proteins α,B2,A expression kinetic | Viral protein expression kinetics | 0,4,8, 12, 16, 20, 22, 24 | 2 |
1D-nanoLC | ESI-ion trap | 16O/18O | FHV proteins α,B2,A expression kinetics | Viral protein expression kinetics | 0,4,8,12, 16, 20, 22, 24 | 3 |
2D-nanoLC | FT-MS | cICAT | FHV/Untargeted cellular proteins | Cellular protein expression changes | 0,8 | 4,5 |
1D-microLC/MS | ESI-TOF | None | FHV/Untargeted small molecules (100–1000 m/z) | Small molecule changes in response to viral infection | 0,12 | 6 |