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Circular dichroism spectra of ALAS- and H282A-ligand complexes. Spectra of ALAS and H282A (A) Holoenzymes; (B) in the presence of 300 μM ALA; (C) in the presence of 200 mM glycine. Spectra were recorded in 20 mM Bis-Tris with 10% glycerol, pH 7.5, at an enzyme concentration of 100 μM.
