Fig. 2. Confirmation of gene and protein levels of 14-3-3 ζ.
(A) RT-PCR confirms the induction of the gene encoding for 14-3-3 ζ in cells transfected with SIRT2 siRNA versus scrambled control siRNA. (B) SIRT2-depleted H9c2 exhibit increased 14-3-3 ζ protein levels compared to controls, as measured by Western immunoblotting. (n = 4 per group. p < 0.05 versus their respective controls)