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. Author manuscript; available in PMC: 2009 Aug 20.
Published in final edited form as: FEBS Lett. 2008 Jul 18;582(19):2857–2862. doi: 10.1016/j.febslet.2008.07.016

Fig. 3. 14-3-3 ζ and BAD protein interactions in response to SIRT2 depletion.

Fig. 3

H9c2 were transfected with SIRT2 siRNA for 48 h. Results were quantified by densitometry (n = 4 per group. p < 0.05 versus scrambled siRNA transfected controls) (A) SIRT2-depleted H9c2 show increased BAD protein associated with 14-3-3 ζ relative to controls. 14-3-3 ζ protein was immunoprecipitated from cell lysates and associated BAD protein detected by Western immunoblotting. (B) SIRT2-depleted H9c2 show increased 14-3-3 ζ protein associated with BAD, relative to controls. BAD protein was immunoprecipitated from cell lysates and associated 14-3-3 ζ protein detected by Western immunoblotting. Immunoprecipitation with (C) 14-3-3 ζ and (D) BAD show increased interaction with the reciprocal protein following 14 hours of anoxia in SIRT2 siRNA cells and a diminution in this interaction in cells overexpressing SIRT2.