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. Author manuscript; available in PMC: 2008 Oct 13.
Published in final edited form as: J Am Chem Soc. 2007 Aug 2;129(33):10220–10228. doi: 10.1021/ja072185g

Figure 3.

Figure 3

Taq polymerase stop assay of the G-rich region of the RET promoter. (A) The sequences of the RET-Pol1 and RET-Pol2 templates used in the Taq polymerase stop assay. The five runs of guanines are labeled I–V. The full-length product, minor stop site, major stop site, and primer site are indicated with arrows. (B) and (C) show the Taq polymerase stop assay with RET-Pol1 and RET-Pol2, respectively, with increasing concentrations of KCl (lane 1, 0 mM KCl; lane 2, 25 mM KCl; lane 3, 50 mM KCl, lane 4, 100 mM KCl). For both (B) and (C), the full-length product, minor stop site, major stop site, and primer site are indicated with arrows on the right. The sequencing reaction for C is shown on the left side of each gel.