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. Author manuscript; available in PMC: 2008 Oct 13.
Published in final edited form as: J Am Chem Soc. 2007 Aug 2;129(33):10220–10228. doi: 10.1021/ja072185g

Figure 4.

Figure 4

Taq polymerase stop assay with the wild-type template (RET-Pol2) and mutant templates. The wild-type template (RET-Pol2) is shown on the top, with arrows indicating the primer site, stop site and full-length product. The four consecutive runs of guanines are underlined in bold, with guanines 3, 8, 13, and 18 indicated. For mutant templates (A3, A8, A13, A18, T3, T8, T13, and T18), the letter refers to the mutated nucleotide and the number refers to the position (3, 8, 13, or 18) on RET-Pol2. For example, A3 means a G-to-A mutation at position 3 on RET-Pol2. The Taq polymerase stop assay was carried out in the absence of (−) or in the presence of 100 mM KCl (+). Sequencing reactions for G and C are shown on the left side of the gel. Lanes 1 and 2 are reactions with the wild-type template (RET-Pol2). Lanes 3–18 are reactions with the different mutant templates. The full-length product, stop site, and primer site are indicated with arrows on the right.