Fig. 5.

The pseudo-(E)LR motif is critical for MIF-mediated monocyte arrest in atherosclerotic carotid arteries. Effect of the pseudo-(E)LR mutants on the arrest of MonoMac6 monocytes in carotid arteries from mif^−/−ldlr^−/− mice fed a Western diet for 6 weeks and comparison with WT MIF. Upon perfusion with calcein AM-labeled monocytes for 10 min, adherent cells per carotid artery were counted. The number of adherent cells after treatment with pseudo-(E)LR mutants as indicated or buffer (control) was normalized in comparison to treatment with WT MIF. Data represent means ± SEM of three independent experiments.