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F-actin and fascin distributions in C2C12 cells adherent on low concentrations of fibronectin. C2C12 cells were allowed to attach for 1 h to substrata coated with 1.25 μg/ml (A and B), 2.5 μg/ml (C and D), 5 μg/ml (E and F), or 20 μg/ml fibronectin (G and H) for 1 h then fixed and stained with TRITC-phalloidin (A, C, E, and G) or with antibody to fascin (B, D, F, and H). Arrows in A–D indicate examples of cells with F-actin- or fascin-containing membrane protrusions. Bar, 10 μm.
