Table 1.
Quantification of cell adhesion to tenascin-C variants
| Cell | Substratum
|
|||
|---|---|---|---|---|
| FN | CEF-TN | TN-190 | TN-ADC | |
| C2C12 | ||||
| % attached cellsa | 100 | 44 | 29 | 31 |
| Ratio spread to attached cells | 0.97 | 0.89 | 0.52 | 0.74 |
| S27 | ||||
| % attached cellsa | 100 | 86 | 60 | 77 |
| Ratio spread to attached cells | 1 | 0.98 | 0.54 | 0.84 |
C2C12 or S27 cells were plated on coverslips coated with 50 nM fibronectin (FN), 50 nM chicken embryo fibroblast tenascin-C (CEF-TN), 50 nM recombinant tenascin-C 190 (TN-190), or 50 nM recombinant tenascin-C ADC (TN-ADC) for 90 min in serum-free medium, and attached cells were then fixed and counted.
a
Numbers of attached cells on fibronectin were set as 100% and the numbers of attached cells on the tenascin-C variants are expressed as percentages relative to this value. Spreading was scored as described in MATERIALS AND METHODS.