Table 2. Comparison of monkeypox virus and variola virus genomes.
A. Genes with known function present in monkeypox virus and absent/fragmented in variola virus
| Gene Ortholog Name | Predicted Function | Present in MPV | Present in VARV |
|---|---|---|---|
| COP-A35R
176 aa |
Unknown | +
176 aa |
- |
| COP-A44L
346 aa |
Hydroxysteriod dehydrogenase | +
346 aa |
Fragment
210 aa: Central/C-term |
| COP-B7R
182 aa |
Virulence, ER resident | +
182 aa |
- |
| COP-B11R
88 aa |
Unknown | +
113 aa |
- |
| COP-B12R
283 aa |
Ser/Thr kinase protein | +
282 aa |
Fragment
104 aa: C-term |
| COP-E7R
166 aa |
Soluble myristylated protein | +
166 aa |
- |
| COP-K1L
284 aa |
Host range protein/Ankyrin protein | +
284 aa |
Fragment
66 aa: N-term 70 aa: C-term |
| COP-K4L
424 aa |
Phospholipase-D protein | +
424 aa |
- |
| BR-18
171 aa |
Unknown | +
176 aa |
- |
| BR-25
668 aa |
Chinese Hamster Ovary Host Range protein | +
660 aa |
Truncated
452 aa: N-term/Central |
| BR-26
63 aa |
Unknown | +
64 aa |
- |
| BR-45
276 aa |
Putative monoglyceride lipase | +
276 aa |
- |
| BR-181
81 aa |
Unknown | +
74 aa |
- |
| BR-197
505 aa |
Schlafen protein | +
503 aa |
- |
| BR-203
225 aa |
Virulence protein | +
221 aa |
- |
| BR-218
193 aa |
Unknown | +
190 aa |
- |
| B. Poxvirus genes with known function absent/fragmented in monkeypox and variola viruses | |||
| Gene Ortholog Name | Predicted Function | Present in MPV | Present in VARV |
| COP-C2L
512 aa |
Kelch protein | Fragment
107 aa: N-term 176 aa: Central 105 aa: C-term |
Fragment
79 aa: Central 221 aa: C-term |
| COP-B_ORF-C
95 aa |
Unknown | - | Fragment
65 aa: C-term |
| BR-08/BR-223
672 aa |
Ankyrin protein | - | Fragment
91 aa: N-term |
| BR-19
796 aa |
Unknown | Fragment
55 aa: N-term 56 aa: N-term 73 aa: C-term |
- |
| BR-158
1284 aa |
CPV A-type inclusion protein | Fragment
696 aa: N-term/Central 54 aa: C-term 67 aa: C-term 75 aa: C-term |
Fragment
702 aa: N-term/Central 65 aa: C-term 96 aa: C-term 192 aa: C-term |
| BR-176
409 aa |
Semaphorin | - | Fragment
122 aa: Central 139 aa: C-term |
| BR-195
197 aa |
Guanylate kinase | - | Fragment
151 aa: Central/C-term |
| BR-209
326 aa |
IL-1β binding protein | Fragment
210 aa: N-term/Central 126 aa: C-term |
- |
| BR-215
557 aa |
Kelch protein | Fragment
70 aa: N-term |
Fragment
70 aa: N-term 88 aa: Central |
| C. Genes with known function present in variola virus but absent/fragmented in monkeypox virus | |||
| Gene Ortholog Name | Predicted Function | Present in MPV | Present in VARV |
| COP-C3L
263 aa |
Inhibitor of complement enzymes | Truncated
216 aa: N-term/Central |
+
263 aa |
| COP-C10L
331 aa |
IL-1β antagonist protein | Fragment
83 aa: N-term |
+
330 aa |
| COP-E3L
190 aa |
IFN-resistance protein | Fragment
153 aa: Central/C-term |
+
192 aa |
| COP-A30.5L
42 aa |
Unknown | - | +
42 aa |
| COP-A49R
162 aa |
Phosphotransferase/Anion transport | - | +
162 aa |
| COP-B_ORF-B
66 aa |
Unknown | - | +
66 aa |
| COP-E_ORF-C
70 aa |
Unknown | - | +
76 aa |
| COP-F14.5L
49 aa |
Unknown | - | +
49 aa |
| COP-K3L
88 aa |
eIF-2α homolog protein | - | +
87 aa |
Abbreviations: aa: amino acids; BR: Cowpox virus strain Brighton Red; C-term: C-terminal fragment; COP: vaccinia virus, strain Copenhagen; CPV: cowpox virus; eIF: eukaryotic initiation factor; ER: endoplasmic reticulum; IFN: interferon; IL-1: Interleukin-1; MPV: central African strain of monkeypox virus Zaire_1979_005; N-term: N-terminal fragment; ORF: open reading frame; Ser/Thr: serine/threonine; VARV: variola virus strain Bangladesh75maj.