Table 1.

Culture Medium

	Isolation Medium	Standard Medium	IVD-like Glucose Medium	IVD-like Osmolarity Medium	IVD-like pH Medium	IVD-like Combined Medium
FCS (%)	5	5	5	5	5	5
Penicillin (U/mL)	100	50	50	50	50	50
Streptoymcin (μg/mL)	100	50	50	50	50	50
Amphotericin (μg/mL)	2.5	1.25	1.25	1.25	1.25	1.25
5 M NaCl 0.4 M KCl (%)				2		2
1 M HCl (%)					3	3
1 M NaOH (%)						0.125
DMEM 4.5 mg/mL Gluc	×	×		×	×
DMEM 1.0 mg/mL Gluc			×			×
Medium characteristics	4.5 mg/mL glucose (=25 mmol/L) 280 mOsm pH 7.6	4.5 mg/mL glucose (=25 mmol/L) 280 mOsm pH 7.6	1.0 mg/mL glucose (=5.56 mmol/L) 280 mOsm pH 7.6	4.5 mg/mL glucose (=25 mmol/L) 485 mOsm pH 7.6	4.5 mg/mL glucose (=25 mmol/L) 280 mOsm pH 6.8	1.0 mg/mL glucose (=5.56 mmol/L) 485 mOsm pH 6.8