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. 2008 Sep 9;99(7):1114–1120. doi: 10.1038/sj.bjc.6604631

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Copyright © 2008 Cancer Research UK

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The effect on invasion of ECE-1 isoforms expressed concomitantly in the epithelial and stromal compartment. The PC-3 cells were transfected with ECE-1a and ECE-1c isoforms using FuGENE-6. Exogenous ECE-1 expression levels were determined using (A) western blot analysis (monoclonal ECE-1 antibody); lane 1, PC-3 cells; lane 2, PC-3 cells expressing ECE-1a; lane 3, PC-3 cells expressing ECE-1c. These cells were then used in a matrigel invasion assay in the presence of stroma transfected with (B) ECE-1a or (C) ECE-1c. Each bar represents the mean value of eight fields counted; ^*P<0.05.