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. 2008 Sep 9;99(7):1114–1120. doi: 10.1038/sj.bjc.6604631

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Copyright © 2008 Cancer Research UK

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The suppressive effect of ECE-1a isoform on invasion-promoting properties of ECE-1c in the presence of stroma. PNT1-a cells were transfected with the ECE-1c isoform using FuGENE-6. Exogenous ECE-1 expression levels were determined using (A) western blot analysis (monoclonal ECE-1 antibody); lane 1, PNT1-a cells; lane 2, PNT1-a cells expressing ECE-1a; lane 3, PNT1-a cells expressing ECE-1c. These cells were then used in a matrigel invasion assay in the presence of stroma. Each bar represents the mean value of eight fields counted; ^*P<0.001.