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. 2008 Sep 9;99(7):1114–1120. doi: 10.1038/sj.bjc.6604631

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Copyright © 2008 Cancer Research UK

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The effect of conditioned media from ECE-1a expressing stromal cells on PC-3 invasion. Stromal cells were transfected with ECE-1a using FuGENE-6 and incubated for 48 h. The media from untreated stroma and ECE-1a expressing stroma was collected and added to the lower well of the invasion chamber. Prostate cancer-3 cells were transfected with ECE-1a and ECE-1c, incubated for 48 h and added to the upper well of the invasion assay. Each bar represents the mean value of eight fields counted.