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. 2008 Sep 16;99(7):1074–1082. doi: 10.1038/sj.bjc.6604676

Figure 4.

Figure 4

Inhibition of EphA2 receptor tyrosine kinase is dose-dependent. Around 90% confluent serum-starved BxPC-3 cells were pretreated with the indicated concentration of dasatinib or 20 μM PP2 for 2 h prior to 2 μg ml−1 ephrinA1-Fc stimulation for 5 min. Cell lysates were immunoprecipitated with anti-EphA2 antibody, analysed by phosphotyrosine (P-Tyr-100) and EphA2 immunoblots. The cell lysates were also analysed by western blot using the indicated antibodies.