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. 2008 Oct 24;4(10):e1000235. doi: 10.1371/journal.pgen.1000235

Figure 1. ZHP-3 shows dynamic localization during meiotic prophase.

Figure 1

(A) Wild-type gonad stained with DAPI and antibodies against SYP-1 and ZHP-3. ZHP-3 largely colocalizes with SYP-1 throughout the gonad. High magnification views of meiotic nuclei in early pachytene (B, C, D and E), mid-pachytene (F and G) and late pachytene (H and I) stained with antibodies against ZHP-3, SYP-1 and HTP-3. The arrows in D and E indicate unsynapsed chromosomes, detected as segments of HTP-3 devoid of SYP-1 and ZHP-3. High magnification views of individual nuclei in late pachytene (J and K) and late pachytene/diplotene (N and O). A single pair of synapsed homologs from each of these nuclei is shown in L, M, P and Q. Note the asymmetric distribution of ZHP-3 in L and focus formation in P on synapsed chromosomes (M and Q). All images are maximum intensity projections of deconvolved 3D stacks. Scale bars represent 4 microns in all figures unless indicated otherwise.