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. 2008 Oct 24;4(10):e1000235. doi: 10.1371/journal.pgen.1000235

Figure 6. Defects in SC disassembly and bivalent structure in zhp-3::gfp mutants.

Figure 6

(A–L) Synaptonemal complex disassembly is disrupted in zhp-3::gfp mutants propagated at 25° and in smo-1 mutants. Bivalents in diakinesis nuclei were stained with antibodies against HTP-3 and SYP-1. At 20°, zhp-3::gfp mutants resemble wild-type animals in that SYP-1 is restricted to the short arm of the diakinesis bivalent. In zhp-3::gfp mutants at 25°, smo-1 single mutants and smo-1; zhp-3::gfp double mutants at 20°, SYP-1 is seen all along the bivalent, similar to the axial element HTP-3. Scale bar indicates 2 microns. (M–P) As homologs desynapse in zhp-3::gfp mutants at 25°, SYP-1 is enriched along what will become the short arm of the bivalent, but is still visible on the long arm. (Q–T) Homologs sometimes appear to separate precociously at diplotene in zhp-3::gfp animals. (U–X) Bivalent structure is disrupted in zhp-3::gfp and smo-1 mutants. Bivalents are stained with DAPI and antibodies against HTP-3. Scale bar indicates 2 microns.