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. 1998 Dec;9(12):3533–3545. doi: 10.1091/mbc.9.12.3533

Table 3.

Results of Lineweaver-Burk analyses of WT and dominant mutant BiP proteins

Vmax Km R2 value
WT 1.65 8 0.999
G274D 0.055 0.72 0.981
G246D 0.058 0.91 0.977
G247D 0.1 2 0.997
K117Q 0.03 0.65 0.981

Steady-state ATPase assays were performed by titrating the amount of nonradioactive ATP from 0.0375 to 100 μM. Vmax is indicated in units of nmol of ATP hydrolyzed·mg−1 of BiP·min−1. Km is indicated in units of μM. The R2 values from the linear regression were obtained as described in MATERIALS AND METHODS.