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. Author manuscript; available in PMC: 2009 Aug 2.
Published in final edited form as: DNA Repair (Amst). 2008 May 20;7(8):1213–1220. doi: 10.1016/j.dnarep.2008.03.025

Figure 2. Accumulation of endogenous DNA damage due to NEIL1-downregulation.

Figure 2

Alkaline Comet analysis of endogenous DNA damage in V79 cells treated with AS or S oligonucleotides as before. The details of Fpg treatment are described in Materials and Methods. The Comet images were taken with a Nikon TE200 epifluorescence UV microscope equipped with a Photometric CoolSNAP Fx camera (Left panel). Tail moments of randomly selected cells (≥50) were analyzed for each treatment with Euclid Comet Analysis software (Euclid Analysis, St. Louis, MO). Data points are the mean (±S.E) of at least three independent experiments (Right panel).