Table-1.
Nature of spontaneous mutations in NEIL1 sense or antisense oligonucleotide-treated V79 cells
| SENSE | ANTISENSE | |||||||
|---|---|---|---|---|---|---|---|---|
| Position | Mutation | Occurrence | AA change | Position | Mutation | Occurrence | AA change | |
| −51 | G>T | 1 | – | −16 | INS-C | 1 | – | |
| +10 | C>G | 2 | R>G | −24 | C>T | 1 | – | |
| +58 | G>T | 1 | D>Y | +1–505 | Rearranged | 1 | Rearranged | |
| +118 | G>T | 1 | G>stop | +2 | T>G | 1 | M>R | |
| +121 | G>A | 1 | V>M | +10 | C>G | 1 | R>G | |
| +133 | A>T | 2 | D>V | +21 | C>T | 2 | S>S | |
| +139 | C>T | 2 | T>I | +28 | INS-T | 1 | Frameshift | |
| −52 to +91 | Del 144 bp | 1 | Frameshift | +47 | G>C | 1 | G>A | |
| +190 | G>C | 1 | A>P | +61 | A>G | 1 | E>E | |
| − 43 to +299 | Del 342 bp | 1 | Frameshift | +82 | T>C | 1 | Y>H | |
| −32 to +280 | Del 312 bp | 1 | Frameshift | +88 | G>A | 1 | D>K | |
| +287 | C>A | 1 | T>N | +133 | A>T | 2 | D>V | |
| +300 | C>G | 1 | I>M | +165 | A>G | 1 | K>K | |
| +359 | A>T | 4 | D>V | +230 | A>G | 1 | D>G | |
| +379 | G>A | 1 | G>R | +236 | T>C | 1 | L>P | |
| +425 | C>A | 1 | T>K | +248 | A>G | 1 | K>R | |
| +442 | T>C | 1 | S>P | +251 | C>T | 1 | A>V | |
| + 460 | A>C | 1 | N>T | +259 | A>G | 1 | R>G | |
| +505–514 | Del-9 bp | 2 | Missing AA | +272 | G>A | 1 | R>K | |
| +505–522 | Del-18 bp | 3 | Missing AA | +274 | T>C | 1 | S>P | |
| +569 | G>C | 1 | G>A | +278 | T>C | 1 | I >P | |
| +602 | A>T | 1 | D>V | +291 | G>C | 1 | D>H | |
| +299 | T>C | 1 | I >C | |||||
| +359 | A>T | 16 | D>V | |||||
| +360 | T>A | 2 | D>A | |||||
| +391 to 407 | Del 17 bp | 2 | Frameshift | |||||
| +413 | A>G | 1 | D>G | |||||
| +421 | A>G | 1 | A>G | |||||
| +427 | A>G | 1 | M>V | |||||
| +430 | C>T | 1 | Q>stop | |||||
| +437 | T>C | 1 | L>P | |||||
| +459 | Del-G | 1 | Frameshift | |||||
| +488 to stop | Rearranged | 1 | Frameshift | |||||
| +493 | G>C | 1 | V>L | |||||
| +504 to 513 | Del-9 bp | 1 | Missing AA | |||||
| +505 to 522 | Del-18 bp | 3 | Missing AA | |||||
| +563 | Del-C | 1 | Frameshift | |||||
| +590 | A>T | 1 | A>V | |||||
| +580 | G>C | 1 | D>H | |||||
| +633 | A>G | 1 | L>R | |||||
| +657 | Del-T | 1 | Frameshift | |||||
| Total | 31 | 62 | ||||||
| Base substitutions | 23 | 49 | ||||||
| A.T | 9 (39 %) | 37 (75.5 %) | ||||||
| G.C | 14 (61 %) | 12 (24.5 %) | ||||||
| Others | 8 | 13 | ||||||
| Not amplified | 8 | 11 | ||||||