Table 2.
Nature of mutations in NEIL1 sense or antisense oligonucleotide-treated V79 cells after GO treatment.
| SENSE | ANTISENSE | |||||||
|---|---|---|---|---|---|---|---|---|
| Position | Mutation | Occurrence | AA change | Position | Mutation | Occurrence | AA change | |
| +21 | C>T | 5 | S>S | 1 to 31 | Rearranged | 1 | Frameshift | |
| +37 | G>C | 1 | D>H | 1 to 505 | Rearranged | 1 | Frameshift | |
| +57 | Del-A | 1 | Frameshift | −30 to +187 | Rearranged | 1 | Frameshift | |
| +72 | C>T | 1 | D>Q | +8 | INS-C | 1 | Frameshift | |
| +118 | G>C | 1 | G>R | +21 | INS-G | 1 | Frameshift | |
| +128 | T>C | 2 | M>T | +21 | C>T | 1 | S>S | |
| +209 | G>A | 1 | G>R | +31 | A>G | 3 | S>G | |
| +289 | G>C | 1 | V>L | +34 | G>A | 1 | D>N | |
| +382 | A>C | 2 | K>Q | +94 | T>G | 1 | L>V | |
| +389 to 405 | Del 17 bp | 2 | Frameshift | +108 | A>T | 1 | I >F | |
| +391 to 407 | Del-17 bp | 3 | Frameshift | +133 | A>T | 4 | D >V | |
| +403 | G>T | 1 | D>Y | +191 | C>T | 1 | A>V | |
| +413 | A>C | 1 | D>A | +202 | C>T | 1 | L>L | |
| +455 | Del-C | 1 | Frameshift | +224 | T>C | 1 | F>L | |
| +459 | Del-G | 1 | Frameshift | +269 | A>G | 1 | D>G | |
| 505–522 | Del-18 bp | 10 | Missing AA | +273 | A>C | 1 | R>S | |
| +464 | Del-C | 1 | Frameshift | +308 | A>G | 1 | K>R | |
| +555 | Del-C | 1 | Frameshift | +355 | G>A | 1 | G>D | |
| +591 | G>A | 1 | E>E | +359 | A>T | 3 | D>V | |
| +559 | T>G | 2 | F>V | +389 to 407 | Del 17 bp | 2 | Frameshift | |
| +599 | G>A | 2 | R>K | +449 | T>C | 1 | V>I | |
| +648 | T>G | 1 | Y>D | +457 | T>A | 1 | Y>N | |
| +648 | T>A | 1 | Y>N | +459 | Del-G | 1 | Frameshift | |
| +657 | Del-T | 1 | Frameshift | +465 | A>C | 1 | K>Q | |
| +505 to 522 | Del-18 bp | 4 | Missing AA | |||||
| Total | 44 | 36 | ||||||
| Base substitutions | 23 | 24 | ||||||
| A.T | 9 (39 %) | 19 (79 %) | ||||||
| G.C | 14 (61 %) | 5 (21 %) | ||||||
| Others | 21 | 12 | ||||||
| Not amplified | 9 | 11 | ||||||