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. Author manuscript; available in PMC: 2009 Aug 19.

Published in final edited form as: Toxicology. 2008 Jun 5;250(1):47–54. doi: 10.1016/j.tox.2008.05.018

The effects of ROS scavengers and anti-oxidant enzymes on arsenical-induced MAPK signaling in UROtsa cells. A) Densitometry analysis of COX-2 protein induction (72 kDa) seen after treatment with 1 μM As(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 4 h. B) Densitometry analysis of COX-2 protein induction (72 kDa) seen after treatment with 50 nM MMA(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 4 h. C) Densitometry analysis of phosphorylated-Src (60 kDa) protein induction seen after treatment with 1 μM As(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 1 h. D) Densitometry phosphorylated-Src protein induction seen after treatment with 50 nM MMA(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 1 h. Values are normalized to GAPDH. All levels are taken relative to control UROtsa with no treatment. (*) marks statistically significant decrease in protein level when compared with arsenical treated group (p≤0.10). (†) Marks statistically significant increase in protein level when compared with control UROtsa (p≤0.10). (N=3) for all treatment groups.

The effects of ROS scavengers and anti-oxidant enzymes on arsenical-induced MAPK signaling in UROtsa cells. A) Densitometry analysis of COX-2 protein induction (72 kDa) seen after treatment with 1 μM As(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 4 h. B) Densitometry analysis of COX-2 protein induction (72 kDa) seen after treatment with 50 nM MMA(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 4 h. C) Densitometry analysis of phosphorylated-Src (60 kDa) protein induction seen after treatment with 1 μM As(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 1 h. D) Densitometry phosphorylated-Src protein induction seen after treatment with 50 nM MMA(III) alone, or in co-treatment with ROS scavengers or anti-oxidant enzymes for 1 h. Values are normalized to GAPDH. All levels are taken relative to control UROtsa with no treatment. (*) marks statistically significant decrease in protein level when compared with arsenical treated group (p≤0.10). (†) Marks statistically significant increase in protein level when compared with control UROtsa (p≤0.10). (N=3) for all treatment groups.