Mutations that affect the ion-coupling capacities of Bs-MotAB and Bs-MotPS. (A) An alignment of a region of Bs-MotB and Bs-MotS (central two lines). The mutational changes introduced into the MotB of Bs-MotAB-LSQ are shown in the top line and those introduced into the MotS of Bs-MotPS are shown in the bottom line. (B) Swimming speed of Bs-MotAB, Bs-MotAB-LSQ, Bs-MotPS, and Bs-MotPS-VAS, as designated by symbols, was assayed as a function of sodium concentration at pH 7.0 (Left) and 9.5 (Right) in TY/20.